Publication date
Clare Rusbridge, F J Salguero, M A David, K M E Faller, J T Brass, R J Guerreiro, A C Richard-Londt, D Grainger, E Head, S G P Brandner, B Summers, J Hardy, M Tayebi
An aged canid with behavioural deficits exhibits blood and cerebrospinal fluid amyloid beta oligomers
Summary
Many of the molecular and pathological features associated with human Alzheimer disease (AD) are mirrored in the naturally occurring age-associated neuropathology in the canine species. In aged dogs with declining learned behaviour and memory the severity of cognitive dysfunction parallels the progressive build up and location of Aβ in the brain. The main aim of this work was to study the biological behaviour of soluble oligomers isolated from an aged dog with cognitive dysfunction through investigating their interaction with a human cell line and synthetic Aβ peptides. We report that soluble oligomers were specifically detected in the dog's blood and cerebrospinal fluid via anti-oligomer-and anti-Aβ specific binders. Importantly, our results reveal the potent neurotoxic effects of the dog's cerebrospinal fluid on cell viability and the seeding efficiency of the cerebrospinal fluid …